MicroRNAs (miRNAs) are small non-coding RNA molecules that play important roles in gene expression and regulation. They have been linked to various diseases and conditions, making them a target for biomarker discovery and diagnostic testing. However, traditional methods of miRNA quantification have limitations such as low sensitivity, high cost, and time-consuming processes. This is where the MinION enabled microRNA quantification comes in - a cutting-edge technology that offers numerous benefits over conventional methods.
High Sensitivity
One of the primary benefits of using MinION technology for miRNA quantification is its high sensitivity. Traditional methods such as qPCR and microarrays have limited dynamic range and often require large amounts of starting material, making it difficult to detect low levels of miRNAs accurately. On the other hand, the nanopore sequencing technology used in MinION enables single-molecule detection with high accuracy, allowing for the detection of even low abundance miRNAs in a sample.
Fast Turnaround Time
Another significant advantage of MinION enabled miRNA quantification is its fast turnaround time. Traditional methods can take days or even weeks to complete, involving multiple steps such as RNA extraction, cDNA synthesis, amplification, and analysis. In contrast, MinION sequencing can provide results within hours, reducing the time required for miRNA analysis significantly. This makes it an ideal tool for time-sensitive research projects or clinical applications.
Cost-Effective
MinION enabled miRNA quantification also offers cost savings compared to traditional methods. Traditional techniques require expensive reagents and equipment, making them costly to perform. In contrast, MinION sequencing only requires a small device called a flow cell and a laptop, making it a more cost-effective option. Additionally, the long sequencing reads provided by MinION reduce the need for multiple sequencing runs, further reducing costs.
Accurate Profiling of miRNA Isoforms
MiRNAs are known to have multiple isoforms, which can have different functions and expression levels. Traditional methods often have difficulty distinguishing between these isoforms, leading to inaccurate profiling of miRNA expression. However, MinION technology allows for accurate identification and quantification of miRNA isoforms due to its long sequencing reads and single-molecule detection capabilities. This provides researchers with a more comprehensive understanding of miRNA biology.
Real-Time Analysis
The ability to perform real-time analysis is another significant benefit of MinION enabled miRNA quantification. Traditional methods require samples to be processed and analyzed in batches, making it difficult to monitor changes in miRNA expression over time accurately. With MinION technology, researchers can analyze samples in real time as they are being sequenced, providing valuable insights into dynamic changes in miRNA expression.
Learn more from a company near you like Yenos Analytical.
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